FIG. 2.

Expression of MOMP affects FN binding by H. ducreyi. Both unlabeled (A) and ¹²⁵I-labeled (B) FN binding assays were repeated, using a panel of mutants constructed in the 35000HP background (Table 1). Asterisks indicate strains with levels of FN binding statistically different (P < 0.05) from the level of FN binding by the wt parent strain 35000HP. Strains not expressing dsrA are indicated with underlining. The results presented in panel A are representative of at least three other similar experiments. Ponceau S staining (panel 5) of the nitrocellulose membrane indicated approximately equal loading of total proteins for all the strains.