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. 2008 Jan 22;76(4):1608–1616. doi: 10.1128/IAI.00994-07

FIG. 3.

FIG. 3.

Either class of DsrA is sufficient to confer FN binding to the non-FN-binding strain H. influenzae KW20 Rd. The same FN binding assays described in the legend to Fig. 1 (unlabeled [A] and 125I-labeled [B] FN binding assays) were used to test FN binding by the H. ducreyi dsrA isogenic mutant FX517 (DsrA negative) and H. influenzae KW20 Rd expressing either an empty vector (pLSKS), pLSKS containing dsrA class I (dsrAI), pLSKS containing dltA, or pLSKS containing dsrA class II (dsrAII). All strains were grown on heme plates. Single asterisks represent statistically significant results (P ≤ 0.01) compared to the H. ducreyi dsrA mutant expressing the empty vector pLSKS, while double asterisks use H. influenzae KW20 Rd expressing the empty vector pLSKS as the comparison strain (P ≤ 0.01). The results presented in panel A are representative of at least three other similar experiments. Ponceau S staining (not shown) of the nitrocellulose membrane indicated approximately equal loading of total proteins for all the strains.