FIG. 5.

DsrA is the major OMP involved in FN binding by other wt H. ducreyi class I strains. FN binding by three other wt H. ducreyi class I strains and their respective dsrA isogenic mutants was determined using the unlabeled FN binding assay described in the legend to Fig. 1A. Results are representative of similar experiments which were done twice. Ponceau S staining (not shown) of the nitrocellulose membrane indicated approximately equal loading of total proteins for all the strains.