TABLE 2.
Plasmids used in this study
| Plasmid | Description or relevant genotype and/or phenotype | Source or reference |
|---|---|---|
| pCR2.1-TOPO | PCR cloning vector; Kanr Ampr | Invitrogen |
| pUC4K | Source of Kanr cassette | 42, 43 |
| pNC40 | Source of CAT cassette | 19 |
| pLSKS | H. ducreyi shuttle vector; Smr | 48 |
| pRSM1791 | Mutagenesis plasmid; β-galactosidase positive; Ampr | 14 |
| pUNCH1256 | dsrA class I from 35000HP with CAT cassette in pRSM1791 | 20 |
| pUNCH1260 | Complete dsrA class I gene from 35000HP in pLSKS | 20 |
| pUNCH1272 | ncaA PCR clone in pCR2.1-TOPO | This study |
| pUNCH1274 | ncaA with CAT cassette in PCR2.1-TOPO | This study |
| pUNCH1275 | ncaA with CAT cassette in pRSM1791 | This study |
| pUNCH1286 | Complete dltA class I gene from 35000HP in pLSKS | 31 |
| pUNCH1296 | Complete dsrA class II gene from CIP 542 in pLSKS | 47 |
| pUNCH1297 | ncaA with Kan cassette in pCR2.1-TOPO | This study |
| pUNCH1298 | ncaA with Kan cassette in pRSM1791 | This study |
| pUNCH1299 | dsrA with Kan cassette insert in pLSKS | This study |
| pUNCH1401 | dsrA with Kan cassette insert in pRSM1791 | This study |
| pUNCH1405 | dsrA with Kan cassette insert in pRSM1791 | This study |