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i.n. infection model. (A) To determine the LD50 for mice infected by the i.n. route, C57BL/6J mice (six or seven per group) were challenged with the indicated numbers of viable F. tularensis LVS bacteria. (B) Five mice per group were challenged i.n. with 3.6 × 103 CFU F. tularensis LVS bacteria, the indicated tissues were collected daily, and total bacterial counts were determined. The horizontal dashed line represents the limit of detection of the assay. d, day.
