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Immunoblot to confirm deletion and overexpression of tapA. Lane 1, A449(pMMB67EH.K); lane 2, tapA::KO(pMMB67EH.K); lane 3, A449(ptapA.K). All strains were grown in TSB with 50 μg/ml kanamycin and 0.05 mM IPTG. The positions of molecular mass standards (in kDa) are indicated on the left.
