TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| A. salmonicida subsp. salmonicida strains | ||
| A449 | Virulent, Cmr | 29 |
| 02-02 | A449 flpA::Km, Cmr Kmr | This study |
| 02-06 | Lab-derived mutant of A449, O side chain negative, surface layer secretor, Cmr | 43 |
| 02-10 | A449 tapA::KO, Cmr Apr | This study |
| 03-01 | A449 flpA::Km tapA::KO, Cmr Kmr Apr | This study |
| P. aeruginosa PAK | Wild type | D. Bradley |
| E. coli strains | ||
| TOP10 | K-12 | Invitrogen |
| DH10B | K-12 for large plasmids | Invitrogen |
| EC100D pir-116 | K-12 pir-116 | Epicenter |
| BW20767 | K-12 pir+, conjugation positive | 28 |
| Plasmids | ||
| pUTkm1 | Cloning vector, Apr Kmr | 13 |
| pCRScript-Amp | oriE1, Apr | Stratagene |
| pAH34 | oriR6Kγ mobRP4, Apr | 28 |
| pKO | pAH34 with tmRNA tag | This study |
| pKO-tapA | Internal fragment of tapA in pKO, in frame with tmRNA tag | This study |
| pGP704 | oriR6Kγ mobRP4, Apr | 21 |
| pJW109-pir | pGP704 with flpA-flanking regions surrounding kanamycin resistance cassette, Kmr Apr | This study |
| pMMB67EH | Low-copy-number, broad-host-range expression vector, mobRSF1010, Apr | 20 |
| pMMB67EH.Km | pMMB67EH with Apr replaced by Kmr | This study |
| ptapA.K | pMMB67EH.Km carrying complete tapA gene | This study |
| pflp1.K | pMMB67EH.Km carrying complete flp-1 gene | This study |
| pBACe3.6 | Large insert vector, Cmr | 19 |
| pAs01a04 | Derivative of pBACe3.6 with 71.2-kb insert carrying the flp locus | This study |