TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Sequence (5′ → 3′)a | Description |
|---|---|---|
| tmRNA tag | GGCCGCAACTAGTTGCAGCAAACGACGAAAACTACGCACTAGCAGCTTAATAACAGAGCT | Coding strand of tmRNA tag |
| tmRNA tag | CTGTTATTAAGCTGCTAGTGCGTAGTTTTCGTCGTTTGCTGCAACTAGTTGC | Noncoding strand of tmRNA tag |
| tapA-1 | ATTGCACTTCCTGCATATCAGACC | Used to amplify internal fragment for knockout |
| tapA-2 | CGGGACCAGTATATATGTGTATGC | Used to amplify internal fragment for knockout |
| tapA-3 | AGGAGAATTCACATGAAGAAGCAATCAGGC | Used for RT-PCR of tap mRNA and to clone complete tapA gene |
| tapA-4 | TATTGGATCCAGAGGTCATGCGTTAGCAG | Used for RT-PCR of tap mRNA and to clone complete tapA gene |
| flpA-1 | CGGCCTTGCATGCCAGCAAGGTTGCG | Used to amplify 5′ flanking region of flpA for knockout |
| flpA-2 | CCAAGATCTGAGCGCTGTCACTTGCCCGCGGC | Used to amplify 5′ flanking region of flpA for knockout and to clone complete flp-1 gene |
| flpA-3 | GCGAGATCTCTGACCACTCCTCTATTTATGTAGTC | Used to amplify 3′ flanking region for knockout |
| flpA-4 | GCTTGCTGGTGAGGATCACATCCACCC | Used to amplify 3′ flanking region for knockout |
| flpA-F | CATATTGATGGCGTGCTGGT | Used for RT-PCR of flp mRNA |
| flpA-R | CACAAAATGGCGAATCCATA | Used for RT-PCR of flp mRNA |
| flpA-5 | GGACGAATTCATGAAGATGCGAGTTTGGG | Used to clone complete flp-1 gene |
| MMB01 | CATCATAACGGTTCTGGC | Used to sequence or amplify insert of pMMB plasmids |
| MMB02 | GAAAATCTTCTCTCATCCGC | Used to sequence or amplify insert of pMMB plasmids |
a
Restriction sites are underlined, and non-sequence-specific regions are indicated by bold type.