FIG. 3.

ERG assessment of retinal function. C57BL/6J mice received intravitreal injections of sterile saline or 500 CFU or 5,000 CFU S. aureus. (A) Representative ERG response to 0.6 cd-s/m² flash intensity at 96 h following 500 CFU or 5,000 CFU S. aureus injection (right and left, respectively). The b-wave amplitude (from the trough of the a-wave [i] to the peak of the b-wave [ii]) was determined, and the ratio of injected eye to contralateral internal control eye was calculated. (B) The 5,000-CFU group lost all retinal function, while the 500-CFU and saline groups displayed only a transient loss of retinal function. The data are expressed as percent retinal function in the experimental left eye compared to the control right eye. Saline, n = 3; 500 CFU, n = 10; 5,000 CFU, n = 10. *, P < 0.001 for 5,000 CFU versus saline and 5,000 CFU versus 500 CFU.