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. 2008 Jan 28;76(4):1518–1526. doi: 10.1128/IAI.01530-07

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FIG. 6. — Protease activity of human aLf with a fluorescent substrate. (A) Effect of substrate concentration on the rate of 0.1 μM aLf-catalyzed hydrolysis of Z-Phe-Arg-AMC. Symbols: □, 0.1 μM aLf; ⋄, 0.1 μM aLf plus 10 μM CaCl₂; ▵, 0.1 μM aLf plus 10 μM FeSO₄; ○, PMSF-treated 0.1 μM aLf; ×, boiled 0.1 μM aLf. The data for PMSF-treated 0.1 μM aLf, 0.1 μM aLf plus 10 μM FeSO₄, and boiled 0.1 μM aLf overlap extensively, as do the data for 0.1 μM aLf and 0.1 μM aLf plus 10 μM CaCl₂. (B) rIsdA inhibits protease activity. Symbols: ⧫, 0.1 μM aLf; ▴, 0.1 μM aLf plus 1 μM rIsdA; ▪, 0.1 μM aLf plus 0.2 μM rIsdA. (C) Anti-IsdA antibodies inhibit IsdA protease inhibitor action. Symbols: ⧫, 0.1 μM aLf; ▴, 0.1 μM aLf plus 1 μM rIsdA; ▪, 0.1 μM aLf plus 0.2 μM rIsdA plus 1:1,000 dilution of anti-IsdA; ×, 0.1 μM aLf plus 0.2 μM rIsdA plus 1:100 dilution of anti-IsdA. The values are the means of three independent experiments.