TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Staphylococcus aureus strains | ||
| Newman | Wild type | 24 |
| SRC105 | isdA::Tn917 Emr in Newman | 18 |
| SRC108 | isdAΔNEAT in Newman | 20 |
| SRC109 | isdAΔC in Newman | 20 |
| SRC150 | ΔsrtA Emr in Newman | 20 |
| Escherichia coli BL21 (DE3) | F−ompT gal [dcm] [lon] hsdSB(rB− mB−) | Novagen |
| Plasmids | ||
| pIsdA | IsdA overexpression vector | 17 |
| pIsdAC | IsdA C domain overexpression vector | 17 |
| pMK4 | E. coli-S. aureus shuttle vector | 59 |
| pSRC001 | rIsdA complementation vector | 17 |