FIG. 5.

Biofilm formation by CECT4999 and its derivatives on glass, polypropylene, and eel mucus measured by determining the absorbance at 540 nm (Multiskan Askcent; Labsystems). The strains used were strains CECT4999 (wild-type strain), 4999-1 (translucent variant), 4999-2 (gne-deficient mutant), 4999-3 (galE-deficient mutant), C4999-2 (gne-complemented strain), and C4999-3 (galE-complemented strain). An asterisk indicates that the value is significantly different from the value for the wild-type strain (P < 0.001).