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. 2008 Feb 4;76(4):1509–1517. doi: 10.1128/IAI.01503-07

FIG. 5.

FIG. 5.

Analysis of the rtxBDE transcript by RT-PCR. Shown is an RT-PCR analysis of RNA isolated from a mid-exponential-phase culture of M06-24/O(pMW0504). The RNA was treated with DNase I (Sigma, St. Louis, MO) and used for the synthesis of cDNA by reverse transcription (SuperScript first-strand synthesis system for RT-PCR; Invitrogen, Carlsbad, CA). The cDNA (lane 1), DNase I-treated RNA (negative control; lane 2), and wild-type genomic DNA (positive control; lane 3) were used as templates for PCR. Molecular size markers (1 kb plus ladder; Invitrogen) and a PCR product are indicated.