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. 2008 Feb 4;76(4):1509–1517. doi: 10.1128/IAI.01503-07

FIG. 6.

FIG. 6.

Identification of a transcription start site of the rtxBDE operon and sequence analysis of the rtxBDE upstream region. (A) The transcription start site was determined by primer extension of the RNA derived from M06-24/O(pMW0504) grown to mid-exponential phase in LBS. Lanes G, A, T, and C represent the nucleotide sequencing ladders of pMW0504. The asterisk indicates the site of transcription start. (B) The transcription start site is indicated by a bent arrow, and the putative promoter regions (−10 and −35) are underlined. The TTG translation initiation codon and putative ribosome binding site (GCAA) are in boldface.