FIG. 3.

Effects of PMB and heating on rPLY-induced IL-1β and IL-6 production. Peritoneal macrophages were stimulated with cholesterol-treated rPLY or LPS for 24 h, and the culture supernatant was then collected. The amounts of IL-1β (A) and IL-6 (B) in the culture supernatant were measured by ELISA. The effects of the addition of 0.5 μg of PMB/ml and heating at 100°C for 1 h were examined. The results are representative of three similar experiments. The data are the means ± standard deviations for three determinations.