TABLE 1.

Cytokine production from splenocytes of mice boosted via the TCI and s.c. routes^a

Treatment group	Level of IFN-γ produced (pg/ml) with the following concn of restimulating antigen (μg/ml):				Level of IL-5b produced (pg/ml) with the following concn of restimulating antigen (μg/ml):				Level of IL-4 produced (pg/ml) with the following concn of restimulating antigen (μg/ml):
	0	0.1	1	10	0	0.1	1	10	0	0.1	1	10
CRM197	5.6 ± 3.8	20.1 ± 12.4	29.5 ± 19.2	119.0 ± 81.1	5.9 ± 5.9	7.5 ± 7.5	37.4 ± 12.8	121.3 ± 37.5	0.7 ± 0.5	2.5 ± 2.3	3.7 ± 3.4	12.3 ± 7.2
CRM197 + CT	79.7 ± 45.9	689.4 ± 483.9	932.2 ± 505.0	929.1 ± 344.4	0.5 ± 0.5	35.6 ± 13.9	60.6 ± 21.5	136.0 ± 24.6	8.6 ± 4.8	40.0 ± 23.9	52.8 ± 25.6	5 6.2 ± 22.8
CRM197 + LTR72	2.9 ± 1.4	80.0 ± 20.2	208.3 ± 37.6	405.5 ± 43.4	0.0 ± 0.0	13.5 ± 6.8	52.5 ± 18.9	148.7 ± 44.0	0.0 ± 0.0	5.9 ± 1.3	15.7 ± 2.8	28.3 ± 8.0
PBS (control)	2.3 ± 2.2	8.6 ± 6.4	12.6 ± 10.8	48.8 ± 27.2	0.0 ± 0.0	2.8 ± 2.8	19.2 ± 19.3	41.0 ± 41.1	0.0 ± 0.0	0.8 ± 0.8	0.7 ± 0.7	3.3 ± 3.3
DTxd (s.c.)	7.3 ± 5.5	39.2 ± 20.8	73.9 ± 47.2	354.3 ± 203.4	12.3 ± 12.3	75.2 ± 53.8	133.1 ± 83.3	317.6 ± 132.3	0.6 ± 0.6	5.0 ± 2.9	8.9 ± 5.7	22.8 ± 11.5

^aSpleen cells isolated from the four groups boosted by TCI were restimulated in the presence of 0.1 to 10 μg/ml CRM₁₉₇. Spleen cells isolated from the group boosted parenterally with DTxd vaccine were restimulated in the presence of 0.1 to 10 μg/ml of purified DTxd. ANOVA was performed for all three cytokines at each dose of restimulating antigen. At the highest dose of restimulating antigen, TCI boost with CRM₁₉₇ with CT as the adjuvant significantly increased splenocyte production of IFN-γ compared to cells isolated from mice in the PBS control group (P < 0.05). Data are the means ± SEMs from three separate spleen cell cultures.

^bIL-5, interleukin 5.