(A) Comparison of MPN136-MPN141 regions in M. pneumoniae strains M129 and S1. The reference strain M129 region contains the MPN139, MPN138, and MPN137 loci. The three-gene operon containing the adhesin P1 gene (MPN141) is located upstream of the MPN139 locus, and the gene encoding a putative permease (ugpE or MPN136) is located immediately downstream of the MPN137 locus. The MPN138/7 fused reading frame (striped bar, deleted sequence) present in clinical strain S1 is surrounded by conserved sequences. The exact positions of PCR-amplified regions and their sizes are indicated. EcoRI restriction sites (E) are indicated together with the positions of two hybridization probes used in Southern analysis (designated “deleted” and “common”). (B) DNA sequence homologies between S1-MPN138/7 and M129-MPN137 and M129-MPN138 reading frames. The fused S1-MPN138/7 region contains the 5′ end of MPN138 and the 3′ end of MPN137. Two nucleotide transitions are indicated, as are 21 nucleotides missing in S1-MPN138/7 (one asterisk). The position of 49 novel nucleotides is indicated by two asterisks.