FIG. 7.

Predictive folding of inlJ 5′-UTR RNAs. inlJ 5′-UTR₁ and 5′-UTR₂ RNAs predicted from the two transcriptional starts (Fig. 1) may fold into structures in which the RBS could be sequestered by a sequence present at the 5′ extremity of the UTR. The energetically most stable structure is shown. The RNA sequence includes the 5′-UTR and 50 bases inside the mRNA coding sequence. Watson-Crick base pairing is indicated in red for cytosine and guanine and in blue for adenine and uracil. Wobble base pairing (guanine with uracil) is indicated in green. (Pairing was done according to Mfold [http://frontend.bioinfo.rpi.edu/applications/mfold/cgi-bin/rna-form1.cgi].).