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IgM levels are not directly correlated with B. burgdorferi burden. MyD88 KO, TLR2 KO, and C57BL/6 mice were inoculated with increased doses of B. burgdorferi (103 to 107) to get a panel of bacterial burdens. Ankle tissues were assessed by quantitative PCR for B. burgdorferi DNA levels in mice sacrificed at 20 days postinfection. Ds, day of sacrifice. Values reflect the numbers of B. burgdorferi fla gene copies normalized per 10,000 copies of a murine gene (gapdh).
