Table 2.

Effect of myristylation and R subunits on the best fit parameters for the anisotropy decay of FM-K16C

FM-labeled configurations	rVF*	rF†	φF,‡ ns	φ,§ ns	τ,¶ ns
Nonmyristylated FM-K16C isoform II
FM-K16C‖ alone	0.10	0.08	1.9  ±  0.1	26  ±  1	3.9
RI:(FM-K16C)2	0.10	0.09	4.6  ±  0.3	133  ±  21	3.9
RII:(FM-K16C)2	0.07	0.14	1.8  ±  0.1	142  ±  19	4.0
Nonmyristylated FM-K16C isoform III
FM-K16C** alone	0.06	0.12	1.9  ±  0.4	28  ±  1	4.2
Myristylated FM-K16C
myr-FM-K16C** alone	0.11	0.13	2.9  ±  0.1	25  ±  1	4.1
RI:(myr-FM-K16C)2	0.11	0.08	3.1  ±  0.2	117  ±  18	4.1
RII:(myr-FM-K16C)2	0.08	0.12	1.8  ±  0.1	134  ±  21	4.2

Anisotropy decays were fit to an empirical nonassociative biexponential anisotropy decay model (Eq. 2) with the Globals Unlimited computer program, and the experimental details are described in Experimental Procedures. Each value represents the mean of at least three determinations, and the average χ² for fitting the data from the various samples ranged between 1.3 and 3.3. 

^*The amplitude of the very fast decay process which equals anisotropy of immobilized fluorescein (0.34) minus r₀, and all the SDs were 0.01. 

^†The amplitude of the very fast decay process, and all the SDs were 0.01. 

^‡Fast rotational correlation time. 

^§Slow rotational correlation time. 

^¶Fluorescein lifetime. 

^‖Phosphorylated at Ser-10, Thr-197, and Ser-338. 

^**Phosphorylation at Thr-197 and Ser-338.