TABLE 1.
Probe sequences designed in-house
| Target organism | Probe sequencea |
|---|---|
| Gram-positive organisms | 5′-FAM-AGGTGGTGCATGGTTGTCGTCAGC-3′-MGB |
| Staphylococcus aureus | 5′-FAM-CCTTTGACAACTCTAGAGATAGAGCCTTCCC-3′-MGB |
| Staphylococcus epidermidis | 5′-TET-AAAACTCTATCTCTAGAGGGGCTAGAGGATGTCAAG-3′-MGB |
| Streptococcus pneumoniaeb | 5′-TET-TCACCTCTGTCCCGAAGGAAAACTCTATCTCTAGA-3′-MGB |
| Streptococcus agalactiae | 5′-FAM-TGCTCCGAAGAGAAAGCCTATCTCTAGGCC-3′-MGB |
| Gram-negative organisms | 5′-VIC-ACAGGTGCTGCATGGCTGTCGTCAGCT-3′-MGB |
| Escherichia coli | 5′-FAM-ACATTCTCATCTCTGAAAACTTCCGTGGATGTC-3′-MGB |
| Neisseria gonorrhoeae | 5′-FAM-TCTCCGGAGGATTCCGCACATGTCAAAA-3′-MGB |
| Uniprobe | 5′-VIC-CACGAGCTGACGACARCCATGCA-3′-MGB |
a
Abbreviations: FAM, 6-carboxyfluorescein; MGB, minor groove binder; TET, tetrachlorofluorescein; VIC, Applied Biosystems dye.
b
The probe may cross-react with the mitis subgroup of the viridans group streptococci (10).