TABLE 3.
Concordant and discordant Uniprobe PCR and type-specific PCR results with clinical synovial fluid samples
| PCR result and sample | Culture resulta | PCR resulta | Organism identification by using the following probeb:
|
Comment(s) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Uni | GP | GN | STAU | STEP | STAG | STPN | ESCO | NEGO | ||||
| Concordant Uniprobe PCR and type-specific PCR results | ||||||||||||
| BAY-051 | STAU | STAU | + | + | − | + | − | − | − | − | − | |
| BAY-062 | STAU | STAU | + | + | − | + | − | − | − | − | − | |
| BAY-133 | STAU | STAU | + | + | − | + | − | − | − | − | − | |
| BAY-160 | STAU | STAU | + | + | − | + | − | − | − | − | − | |
| BTW-J0003 | STAU | STAU | + | + | − | + | − | − | − | − | − | |
| BTW-J0026 | STAU | STAU | + | + | − | + | − | − | − | − | − | |
| BTW-J0039 | STAU | STAU | + | + | − | + | − | − | − | − | − | |
| BTW-J0077 | STAU | STAU | + | + | − | + | − | − | − | − | − | |
| BTW-J0078 | STAU | STAU | + | + | − | + | − | − | − | − | − | |
| BTW-J0084 | STAU | STAU | + | + | − | + | − | − | − | − | − | |
| BTW-J0094 | STAU | STAU | + | + | − | + | − | − | − | − | − | |
| BTW-J0096 | STAU | STAU | + | + | − | + | − | − | − | − | − | |
| BTW-J0115 | STAU | STAU | + | + | − | + | − | − | − | − | − | |
| BTW-J0079 | STEP | STEP | + | + | − | − | + | − | − | − | − | |
| BTW-J0098 | STEP | STEP | + | + | − | − | + | − | − | − | − | |
| BTW-J0102 | STAG | STAG | + | + | − | − | − | + | − | − | − | |
| Discordant Uniprobe PCR and type-specific PCR results | ||||||||||||
| BTW-J0069 | STAU | STEP | + | + | − | − | + | − | − | − | − | Sequenced; no quality data |
| BTW-J0019 | STAU | Negative | − | − | − | − | − | − | − | − | − | Grew in broth after 3 days |
| BAY-157 | Group G streptococcusc | No probe | + | + | − | − | − | − | − | − | − | Sequenced; group G streptococcus |
| BTW-J0030 | Viridans group streptococcid | Streptococcus pneumoniae | + | + | − | − | − | − | + | − | − | Sequenced; Streptococcus pneumoniae |
| BTW-J0031 | Viridans group streptococcid | Streptococcus pneumoniae | + | + | − | − | − | − | + | − | − | Sequenced; Streptococcus pneumoniae |
| BTW-J0049 | Negative | STEP | + | + | − | − | + | − | − | − | − | Sequenced; no quality data |
| BTW-J0086 | Negative | Positive | + | + | + | − | − | − | − | − | − | Sequenced; no quality data |
| BTW-J0101 | Negative | Positive | + | + | + | − | − | − | − | − | − | Sequenced; no quality data |
a
Abbreviations: STAU, S. aureus; STEP, S. epidermidis; STAG, S. agalactiae.
b
Abbreviations: Uni, Uniprobe; GP, gram-positive organisms; GN, gram-negative organisms; STAU, S. aureus; STEP, S. epidermidis; STAG, S. agalactiae; STPN, S. pneumoniae; ESCO, E. coli; NEGO, N. gonorrhoeae. Symbols: +, positive; −, negative.
c
BAY-157 was reported as group B; it was replated, recultured, and identified as group G streptococcus. Sequencing confirmed it as group G streptococcus.
d
BTW-J0030 and BTW-J0031 were reported as viridans group streptoccus. It was positive for our Streptoccus pneumoniae probe, and sequencing confirmed it as Streptoccus pneumoniae.