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. 2003 Nov;77(21):11324–11331. doi: 10.1128/JVI.77.21.11324-11331.2003

FIG. 1.

FIG. 1.

Characterization of gradient-purified FFV. FFV was first concentrated by ultracentrifugation and then loaded onto a 20 to 66% sucrose gradient. Four hundred-microliter fractions were drawn from the bottom. (A) Ten microliters of each fraction was diluted with 990 μl of Dulbecco's modified Eagle's minimal essential medium and used for the determination of viral infectivity on CrFK/LTR(FFV)lacZ cells. (B) Twenty microliters of each fraction was loaded onto a sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel, which was blotted, and stained with a rabbit anti-FFV Gag serum. The migration of molecular mass markers is indicated. (C) DNA was extracted from each fraction and analyzed by Southern blotting with a labeled FFV pol gene probe.