FIG. 1.

RCA results monitored by the RotorGene 3000 real-time PCR machine (Corbett Research). The accumulation of double-stranded DNA was detected by staining with Sybr green I. Positive signals (labeled as “positive signal”) are shown as exponential increases in fluorescence signal. The experiment was conducted using the T. mentagrophytes-specific RCA probe (Tm-ITS1) and tested on eight T. mentagrophytes and other dermatophyte isolates. Ligation-mediated RCA with matched templates of T. mentagrophytes containing the targeted SNPs produced positive signals. In contrast, other species (e.g., T. tonsurans, T. rubrum, T. violaceum, E. floccosum, and M. canis) showed an absence of signal (labeled as “negative signal”).