TABLE 2.
Target genes and sequences of virus specific regions of the MLPA probes
| Virus | Gene | MLPA oligonucleotide | Sequence of virus-specific regions of MLPA probe (5′-3′) |
|---|---|---|---|
| InfA | Matrix protein gene (M1) | Synthetic | CCATGCACGCTCACCGTGCCCAGTGAGCGAGG |
| Long | ACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTCAATGGGAATG | ||
| InfB | Matrix protein gene (M1) | Synthetic | GACAGAAGATGGAGAAGGCAAAGCAGA |
| Long | ACTAGCAGAAAAATTACACTGTTGGTTCGGTGGGAAAGAA | ||
| InfA H5N1 | Matrix protein gene (M1) | Synthetic | CIGTIACTACIGAAGTGGCTTTTGGCCTA |
| Long | GTGTGTGCCACTTGTGAGCAGATTGCAGATTCACAGCA | ||
| RSVA | Major nucleocapsid protein gene (N) | Synthetic | GGCTCTTAGCAAAGTCAAGTTGAATGATACACTC |
| Long | AACAAAGATCAACTTCTGTCATCCAGCAAATACACCATCCAACGGA | ||
| RSVB | Major nucleocapsid protein gene (N) | Synthetic | GTCCAGGTTAGGAAGGGAAGACACTATAAAGATACTT |
| Long | AAAGATGCTGGATATCATGTTAAAGCTAATGGAGTAGATATAACAA | ||
| PIV-1 | Hemagglutinn-neuraminidase gene (HN) | Synthetic | CAGGATGTGTTAGACTACCTTCATTATCAATTGGT |
| Long | GATGCAATATATGCGTATTCATCAAACTTAATCACTCAAGGATGTGC | ||
| PIV-2 | Hemagglutinn-neuraminidase gene (HN) | Synthetic | CAGGACTATGAAAACCATTTACCTAAGTGATGG |
| Long | AATCAATCGCAAAAGCTGTTCAGTCACTGCTATACCAGGAGGTTGT | ||
| PIV-3 | Hemagglutinn-neuraminidase gene (HN) | Synthetic | CCATCTGTTGGACCAGGGATATACTACAAA |
| Long | GGCAAAATAATATTTCTCGGGTATGGAGGTCTTGAACATCCAA | ||
| PIV-4 | Hemagglutinn-neuraminidase gene (HN) | Synthetic | GATTATACATTAACTATTAATCCAAGATCTGGCTGGGATG |
| Long | ACATCAAGATCAGAGCTTATAGAGCATTATCCAGAGATTTGC | ||
| Rhinovirus | 5′ untranslated region polyprotein gene (PP) | Synthetic | GACAIGGTGTGAAGAGCCCCGTGTGCTIIIITTGAITCCTCC |
| Long | GGCCCCTGAATGTGGCTAACCTTAACCC | ||
| Cor-229E | Nucleocapsid protein gene (NP) | Synthetic | CCCAGAGACCTTGACCACAACTTTGGAAGT |
| Long | GCAGGTGTTGTGGCCAATGGTGTTAAAGCTAAAGGCTATC | ||
| Cor-OC43 | Nucleocapsid protein gene (NP) | Synthetic | CTATTGCACCAGGAGTCCCAGCTACTGAA |
| Long | GCTAAGGGGTACTGGTACAGACACAACAGACGTTCTTTTAAAAC | ||
| Cor-NL63 | Nucleocapsid protein gene (NP) | Synthetic | CCAGTCGAAGTCACCTAGTTCTTCTGGTA |
| Long | CTTCCACTCCTAAGAAACCTAATAAGCCTCTTTCTCAACCCAGGGCT | ||
| hMPV | Nucleocapsid protein gene (NP) | Synthetic | GCTCATGCATCCCACAAAATCAGAGGCCTTCAGCACCAG |
| Long | ACACACCAATAATTTTATTATGTGTAGGTGCCTTAATATTCACTAAACTAGCATCAA | ||
| Adenovirus | Hexon gene (H) | Synthetic | GAAGTTTTCGACGTGGTCCGAGTGCACCAGCCGCACCGC |
| Synthetic | GAAGTTTTCGACGTGGTCAGTGTGCATCAGCCACACCGC | ||
| Long | GGCGTCATCGAGGCCGTCTACCTGCGCACACCGTTCTC | ||
| EMC (IAC) | Polyprotein gene (PP) | Synthetic | GCAGTCAGGTGAAGCACCCAGACTTGCCTCCTTGT |
| Long | GAGAGGCCGCACCTTGGTAGTAAATAGACACATGGCCGAGT |