TABLE 2.
Primers used in this study for the identification and characterization of the MAC isolates
| Name or target | Directiona | Sequence (5′ to 3′) | Reference(s) |
|---|---|---|---|
| Mycgen-F/R | F | AGAGTTTGATCCTGGCTCAG | 5, 43 |
| R | TGCACACAGGCCACAAGGGA | ||
| Mycint | F | CCTTTAGGCGCATGTCTTTA | 43 |
| R | TGCACACAGGCCACAAGGGA | ||
| IS901 | F | GCAACGGTTGTTGCTTGAAA | 20 |
| R | TGATACGGCCGGAATCGCGT | ||
| IS1245 | F | GCCGCCGAAACGATCTAC | 13 |
| R | AGGTGGCGTCGAGGAAGA | ||
| IS1311 | F | GCGTGAGGCTCTGTGGTGAA | 23 |
| R | ATGACGACCGCTTGGGAGAC | ||
| ITS | F | TTGTACACACCGCCCGTCA | 31 |
| R | TCTCGATGCCAAGGCATCCACC | ||
| hsp65 3′ fragment | F | CGGTTCGACAAGGGTTACAT | 39 |
| R | ACGGACTCAGAAGTCCATGC | ||
| hsp65 5′ fragmentb | F | ACCAACGATGGTGTGTCCAT | 35 |
| R | CTTGTCGAACCGCATACCCT |
a
F, forward; R, reverse.
b
Telenti fragment.