Skip to main content
. 2008 Feb 13;82(8):3882–3893. doi: 10.1128/JVI.02622-07

FIG. 4.

FIG. 4.

Long-distance RNA-RNA interaction required for transcriptional activation of the N gene. (A) Scheme showing the genetic structure of TGEV-derived replicons. The 9-nt sequence segments are represented by small white boxes within the proximal and distal 5′ TRS-N regions. The scheme of different mutant replicons generated to analyze the functional relevance of the putative RNA-RNA interaction is shown at the bottom, and their names are indicated to the left. The sequences of the 9-nt motif mutants located within the proximal and distal 5′ TRS-N regions are represented in smaller black boxes. Asterisks represent nucleotides identical to those in E2-TRS-N. Potential base pairing between proximal and distal sequences is shown in the right column by vertical lines. See the legend to Fig. 3 for more details. UTR, untranslated region. (B) Quantification of sg mRNA N by real-time RT-PCR. The amounts of sg mRNA N expressed by each mutant replicon are shown relative to that of sg mRNA 7. The data are the averages of four independent experiments performed in triplicate in each case. Error bars represent standard deviations.