TABLE 1.

Oligonucleotides used for site-directed mutagenesis

Mutant	Oligonucleotide	Oligonucleotide sequence (5′ → 3′)a
E-TRS-N	N-800 AscI VS	TTGGCGCGCCACTCCTTGCTTGAACTAAACAAAATG
	3′-AscI RS	TTGGCGCGCCTTAGTTCGTTACCTCATCAATTATC
TRS-N	N-120 AscI VS	TTGGCGCGCCTTGAAAGCAAGTAGTGCGACTGG
	3′-AscI RS
E-TRS-GFP	E-800 AvrII VS	TTCCTAGGACTCCTTGCTTGAACTAAACAAAATG
	GFP 5′ RS	CAGCTCCTCGCCCTTGCTCACCATTTAGAAGTTTAGTTA
	GFP 5′ VS	ACATATGGTATAACTAAACTTCTAAATGGTGAGCAAGGGC
	3′AscI GFP RS	AAGGCGCGCCGGAAGAAAGCGAAAGGAGCGGGCGC
E-TRS3a-N	E-800 AvrII VS
	E-120 AvrII RS	AACCTAGGCTTCTTGCCAACAAGTGTGTAGACAATAGTC
E1-TRS-N	E-800 AvrII VS
	E-460 AvrII RS	AACCTAGGGACACTTGGTATTCCGAGTATGCATTAAAAATC
E2-TRS-N	E-740 AvrII VS	TTCCTAGGTGGAACTTCAGCTGGTCTATAATATTGATC
	E-160 AvrII RS	AACCTAGGCATAGCTTCTTCCTAATGCACTAACGCAAAG
E3-TRS-N	E-460 AvrII VS	TTCCTAGGCAGATATGTAATGTTCGGCTTTAGTATTGC
	E-120 AvrII RS
M1-TRS-N	Rep Mut 2.1 VS	GTCCAGATTACATATGTTCGGCTTTAGTAT
	Rep Mut 2.1 RS	CCGAACATATGTAATCTGGACACTTGGTAT
M2-TRS-N	Rep Mut 2.2 VS	AAAATTATATGTAATGGTATAACTAAACTT
	Rep Mut 2.2 RS	TATACCATTACATATAATTTTTCTTGCTCA
M4-TRS-N	Rep Mut 2 A1G VS	GTGTCCAGGTATGTAATGTTCGGCTTTAGTA
	Rep Mut 2 A1G RS	AGCCGAACATTACATACCTGGACACTTGGTATTC
M5-TRS-N	Rep Mut 2 A1C VS	GTGTCCAGCTATGTAATGTTCGGCTTTAGTA
	Rep Mut 2 A1C RS	AGCCGAACATTACATAGCTGGACACTTGGTATTC
M6-TRS-N	Rep Mut 2 A1C-U9C VS	GTGTCCAGCTATGTAACGTTCGGCTTTAGTA
	Rep Mut 2 A1C-U9C RS	AGCCGAACGTTACATAGCTGGACACTTGGTATTC
M7-TRS-N	Rep Mut 2 CC-U9C VS	GTGTCCCCATACGTAATGTTCGGCTTTAGTA
	Rep Mut 2 CC-U9C RS	AGCCGAACATTACGTATGGGGACACTTGGTATTC

^aThe mutated nucleotides are shown in boldface. The restriction endonuclease sites used for cloning are underlined (AvrII, CCTAGG; AscI, GGCGCGCC).