TABLE 1.
Genes upregulated more than 10-fold in all of the HTLV-1-carrying T-cell lines compared with the HTLV-1-negative T-cell line MOLT-4a
| Accession no. | Symbol | Relative expression level (fold change)
|
Gene product | ||
|---|---|---|---|---|---|
| S1T | MT-2 | M8166 | |||
| NM_138410 | CKLFSF7 | 1,886.6 ± 188.7 | 293.0 ± 31.0 | 113.3 ± 19.9 | CKLF-like MARVEL transmembrane domain containing 7 isoform a |
| NM_001252 | CD70 | 1,375.3 ± 137.5 | 2,635.3 ± 263.5 | 2,594.8 ± 259.5 | Tumor necrosis factor ligand superfamily, member 7 |
| NM_005572 | FPL | 769.8 ± 88.2 | 822.0 ± 155.1 | 1,361.6 ± 136.2 | Lamin A/C isoform 2 |
| NM_004364 | CEBP | 539.9 ± 191.3 | 87.2 ± 8.7 | 56.9 ± 19.8 | CCAAT/enhancer binding protein α |
| NM_022555 | HLA-DR3B | 474.0 ± 93.8 | 14.4 ± 4.9 | 54.0 ± 45.3 | MHC II, DRβ3 precursor |
| NM_004364 | CEBP | 468.8 ± 144.7 | 72.8 ± 7.3 | 47.1 ± 17.4 | CCAAT/enhancer binding protein alpha |
| NM_002304 | NM_002304 | 336.0 ± 78.3 | 172.3 ± 36.6 | 135.3 ± 16.4 | |
| NM_002166 | GIG8 | 331.2 ± 33.1 | 265.4 ± 31.0 | 47.3 ± 24.0 | Inhibitor of DNA binding 2 |
| NM_024644 | FLJ21802 | 325.1 ± 32.5 | 108.8 ± 18.0 | 107.3 ± 35.1 | Chromosome 14 open reading frame 169 |
| NM_015392 | CAB | 275.4 ± 27.5 | 130.9 ± 13.1 | 23.4 ± 2.3 | Neural proliferation 1, differentiation and control |
| NM_014580 | GLUT8 | 221.3 ± 22.1 | 93.3 ± 13.0 | 116.4 ± 11.8 | Solute carrier family 2, (facilitated glucose transporter) member 8 |
| NM_024710 | TMEM101 | 217.8 ± 37.6 | 228.6 ± 54.0 | 245.8 ± 66.3 | Isochorismatase domain containing 2 |
| NM_015691 | BM042 | 204.4 ± 20.4 | 80.5 ± 8.0 | 34.5 ± 7.7 | WWC family member 3 |
| NM_015892 | GALNAC4S-6ST | 178.9 ± 18.2 | 40.5 ± 6.8 | 37.0 ± 3.8 | B-cell RAG-associated protein |
| NM_001017535 | NR1I1 | 140.8 ± 42.1 | 178.1 ± 81.8 | 109.3 ± 66.6 | Vitamin D (1,25-dihydroxyvitamin D3) receptor |
| NM_033518 | SN2 | 113.5 ± 26.6 | 31.5 ± 11.6 | 58.5 ± 22.2 | Amino acid transport system N2 |
| NM_181078 | NILR | 112.5 ± 11.3 | 127.1 ± 25.3 | 178.5 ± 17.8 | IL-21 receptor precursor |
| NM_012081 | ELL2 | 112.4 ± 23.2 | 52.9 ± 5.3 | 19.7 ± 2.0 | Elongation factor 2, RNA polymerase II |
| ENST00000297871 | 102.3 ± 10.2 | 82.4 ± 8.2 | 95.9 ± 9.6 | ||
| NM_004737 | MDC1D | 97.8 ± 15.4 | 15.8 ± 1.7 | 106.7 ± 26.4 | Like glycosyltransferase |
| NM_139346 | AMPH2 | 83.6 ± 8.4 | 129.0 ± 12.9 | 119.6 ± 12.6 | Bridging integrator 1 isoform 8 |
| NM_022121 | THW | 74.0 ± 7.4 | 29.3 ± 3.0 | 65.1 ± 6.6 | PERP, TP53 apoptosis effector |
| NM_014580 | GLUT8 | 71.7 ± 7.2 | 26.0 ± 3.1 | 31.0 ± 3.1 | Solute carrier family 2, (facilitated glucose transporter) member 8 |
| NM_004350 | AML2 | 67.8 ± 6.8 | 24.9 ± 2.5 | 61.0 ± 6.1 | Runt-related transcription factor 3 isoform 1 |
| NM_012465 | KIAA0932 | 65.1 ± 13.3 | 24.5 ± 7.7 | 20.2 ± 2.7 | Tolloid-like 2 |
| NM_000447 | AD4 | 64.6 ± 9.4 | 87.4 ± 19.1 | 58.6 ± 8.1 | Presenilin 2 isoform 1 |
| NM_016010 | CGI-62 | 63.1 ± 6.3 | 24.6 ± 2.5 | 23.5 ± 2.4 | Hypothetical protein LOC51101 |
| NM_000447 | AD4 | 62.2 ± 12.0 | 90.9 ± 19.7 | 57.1 ± 11.1 | Presenilin 2 isoform 1 |
| NM_000786 | LDM | 60.9 ± 8.1 | 59.2 ± 11.6 | 63.0 ± 6.3 | Cytochrome P450, family 51 |
| NM_005658 | EBI6 | 54.6 ± 35.4 | 85.3 ± 14.2 | 165.7 ± 71.1 | TNF receptor-associated factor 1 |
| NM_025195 | C8FW | 53.8 ± 19.9 | 170.5 ± 17.1 | 29.9 ± 3.7 | G-protein-coupled receptor-induced protein |
| NM_002306 | GAL3 | 52.1 ± 5.2 | 133.4 ± 14.6 | 81.8 ± 11.0 | Galectin 3 |
| AK057088 | AK057088 | 51.8 ± 5.2 | 11.4 ± 1.1 | 20.4 ± 2.0 | |
| NM_000786 | LDM | 50.9 ± 7.1 | 49.2 ± 11.5 | 52.6 ± 5.3 | Cytochrome P450, family 51 |
| NM_000958 | EP4 | 50.3 ± 10.5 | 22.7 ± 4.6 | 43.0 ± 7.0 | Prostaglandin E receptor 4, subtype EP4 |
| NM_177925 | MGC921 | 48.9 ± 4.9 | 134.3 ± 13.4 | 132.5 ± 13.3 | H2A histone family, member J isoform 1 |
| NM_003254 | EPA | 48.0 ± 15.7 | 20.1 ± 2.7 | 10.9 ± 3.2 | Tissue inhibitor of metalloproteinase 1 precursor |
| NM_018094 | GST2 | 47.4 ± 5.0 | 68.6 ± 6.9 | 68.1 ± 7.2 | Peptide chain release factor 3 |
| BC018597 | TEX264 | 43.8 ± 4.4 | 51.8 ± 5.2 | 74.4 ± 10.0 | |
| THC2440229 | 43.7 ± 9.9 | 13.5 ± 4.2 | 11.3 ± 3.1 | ||
| NM_002200 | IRF5 | 43.7 ± 5.3 | 229.9 ± 23.0 | 48.9 ± 7.3 | Interferon regulatory factor 5 isoform a |
| NM_014178 | amisyn | 38.5 ± 3.9 | 200.4 ± 32.5 | 30.6 ± 3.1 | Amisyn |
| NM_014417 | JFY1 | 38.4 ± 9.4 | 82.8 ± 14.8 | 118.3 ± 49.0 | BCL2 binding component 3 |
| NM_000199 | HSS | 37.6 ± 4.7 | 10.5 ± 1.0 | 12.8 ± 1.4 | N-Sulfoglucosamine sulfohydrolase (sulfamidase) |
| AK090416 | RXRA | 37.3 ± 8.1 | 12.3 ± 4.1 | 27.9 ± 10.2 | FLJ00318 protein |
| NM_000418 | CD124 | 37.2 ± 6.2 | 43.8 ± 4.4 | 29.6 ± 9.7 | Interleukin 4 receptor α chain isoform a precursor |
| NM_015111 | N4BP3 | 36.5 ± 5.5 | 14.2 ± 1.4 | 27.5 ± 2.9 | Nedd4 binding protein 3 |
| NM_015459 | DKFZP564J0863 | 34.8 ± 6.6 | 26.2 ± 2.6 | 18.5 ± 5.4 | Hypothetical protein LOC25923 |
| NM_018664 | SNFT | 34.7 ± 3.5 | 24.6 ± 2.5 | 11.7 ± 2.5 | Jun dimerization protein p21SNFT |
| NM_013385 | CYT4 | 34.1 ± 3.4 | 16.2 ± 4.7 | 107.6 ± 34.4 | Pleckstrin homology, Sec7, and coiled-coil |
| NR_002323 | TUG1 | 33.4 ± 3.3 | 30.3 ± 3.0 | 30.1 ± 5.2 | domains 4 |
| BC004219 | MGC4604 | 33.1 ± 3.3 | 21.0 ± 2.1 | 13.0 ± 3.2 | 1-Acylglycerol-3-phosphate O-acyltransferase 3 |
| BC035647 | HLA-B | 33.0 ± 6.3 | 52.4 ± 5.2 | 36.3 ± 5.7 | |
| NM_006035 | MRCKB | 32.0 ± 3.2 | 65.7 ± 6.6 | 74.0 ± 7.4 | CDC42-binding protein kinase β |
| NM_001919 | CD79A | 31.8 ± 3.2 | 15.6 ± 1.7 | 27.2 ± 4.0 | Dodecenoyl-coenzyme A δ isomerase precursor |
| BQ189193 | ICOSLG | 31.8 ± 14.2 | 30.1 ± 3.8 | 72.7 ± 7.3 | |
| THC2401087 | 31.2 ± 3.1 | 42.5 ± 4.2 | 14.6 ± 4.0 | ||
| NM_001852 | MED | 30.7 ± 6.6 | 172.2 ± 33.3 | 52.9 ± 5.3 | α2 type IX collagen |
| NM_004357 | GP27 | 30.0 ± 4.4 | 34.0 ± 3.4 | 14.5 ± 4.5 | CD151 antigen |
| NM_006509 | I-REL | 29.9 ± 3.0 | 65.0 ± 6.5 | 43.5 ± 5.1 | Reticuloendotheliosis viral oncogene homolog B |
| AK096677 | AK096677 | 29.2 ± 3.8 | 29.7 ± 7.2 | 73.3 ± 36.4 | |
| NM_024758 | FLJ23384 | 27.7 ± 4.5 | 23.1 ± 5.6 | 13.6 ± 2.1 | Agmatine ureohydrolase (agmatinase) |
| AK021777 | FLJ00205 | 25.8 ± 2.6 | 16.8 ± 1.7 | 19.9 ± 2.0 | GalNAc transferase 10 isoform b |
| NM_001953 | TP | 25.2 ± 2.5 | 43.2 ± 4.3 | 30.8 ± 5.9 | Endothelial cell growth factor 1 (platelet-derived) |
| NM_020731 | AHH | 24.8 ± 5.3 | 10.1 ± 1.6 | 99.4 ± 42.5 | Arylhydrocarbon receptor repressor |
| NM_002413 | GST2 | 24.6 ± 5.6 | 45.4 ± 4.6 | 51.5 ± 5.2 | Microsomal glutathione S-transferase 2 |
| NM_014383 | Rog | 24.3 ± 2.4 | 41.1 ± 4.1 | 12.5 ± 3.8 | Testis zinc finger protein |
| NM_003928 | MGC117411 | 24.2 ± 2.4 | 61.8 ± 7.1 | 35.8 ± 3.6 | CAAX box 1 |
| BX362492 | BX362492 | 23.1 ± 4.0 | 26.3 ± 2.8 | 42.5 ± 11.0 | |
| NM_033375 | myr2 | 20.8 ± 2.9 | 45.4 ± 8.2 | 48.5 ± 8.3 | Myosin IC |
| NM_213589 | LPD | 20.6 ± 2.3 | 14.9 ± 3.5 | 24.8 ± 3.1 | Ras association and pleckstrin homology domains 1 isoform 2 |
| NM_004838 | HOMER-3 | 20.3 ± 2.0 | 193.6 ± 23.6 | 84.5 ± 15.8 | Homer 3, neuronal immediate early gene |
| A_23_P370707 | 18.2 ± 7.7 | 42.1 ± 4.2 | 30.1 ± 7.0 | ||
| AB033060 | AHH | 17.9 ± 3.9 | 12.2 ± 1.2 | 114.8 ± 22.4 | Arylhydrocarbon receptor repressor |
| BC024020 | VMP1 | 17.6 ± 2.6 | 39.6 ± 4.0 | 22.8 ± 6.0 | Transmembrane protein 49 |
| NM_023076 | FLJ23360 | 17.6 ± 3.1 | 21.2 ± 4.0 | 20.5 ± 2.8 | Hypothetical protein LOC65259 |
| AK097976 | DLEU2 | 17.2 ± 3.1 | 22.5 ± 5.3 | 13.6 ± 8.3 | |
| NM_003842 | DR5 | 16.7 ± 1.7 | 32.9 ± 4.0 | 37.2 ± 3.7 | TNF receptor superfamily, member 10b isoform 1 precursor |
| NM_024646 | ZYG11 | 16.5 ± 1.7 | 11.1 ± 1.1 | 15.1 ± 1.5 | Zyg-11 homolog B |
| AK092921 | HLA-B | 16.3 ± 1.6 | 25.7 ± 2.6 | 19.9 ± 2.0 | |
| NM_022152 | RECS1 | 16.2 ± 1.6 | 13.6 ± 2.9 | 14.7 ± 3.0 | Transmembrane BAX inhibitor motif containing 1 |
| AA451906 | BIN1 | 15.4 ± 1.5 | 18.2 ± 2.0 | 17.3 ± 1.7 | |
| NM_018370 | DRAM | 15.3 ± 2.5 | 80.9 ± 14.7 | 41.6 ± 10.8 | Damage-regulated autophagy modulator |
| NM_005514 | HLA B | 15.2 ± 1.7 | 32.3 ± 3.2 | 28.0 ± 4.9 | MHC I, B |
| THC2403644 | 14.9 ± 4.4 | 17.5 ± 5.9 | 15.0 ± 5.0 | ||
| NM_000152 | LYAG | 14.7 ± 1.5 | 15.8 ± 2.4 | 13.0 ± 2.1 | Acid α-glucosidase preproprotein |
| ENST00000355804 | 14.4 ± 1.4 | 23.7 ± 2.8 | 13.1 ± 1.7 | ||
| NM_001613 | ACTSA | 14.4 ± 1.6 | 35.3 ± 5.4 | 22.3 ± 2.3 | α2 actin |
| NM_018370 | DRAM | 14.3 ± 2.3 | 73.3 ± 12.4 | 33.9 ± 7.7 | Damage-regulated autophagy modulator |
| A_24_P101771 | 14.0 ± 1.5 | 21.3 ± 2.2 | 19.3 ± 3.3 | ||
| NM_017789 | SEMAI | 13.9 ± 1.7 | 20.4 ± 2.5 | 33.9 ± 13.8 | Semaphorin 4C |
| NM_002502 | LYT10 | 13.3 ± 4.3 | 15.4 ± 1.0 | 45.9 ± 2.8 | Nuclear factor of κ light polypeptide gene enhancer in B cells 2 (p49/p100) |
| NM_031419 | IKBZ | 12.6 ± 4.6 | 37.1 ± 4.9 | 134.0 ± 62.5 | Nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor, ζ isoform b |
| NM_015516 | TSK | 12.5 ± 1.2 | 12.7 ± 1.3 | 12.2 ± 1.2 | Tsukushi |
| CR594843 | HLA-B | 12.2 ± 1.2 | 11.5 ± 1.2 | 10.1 ± 1.9 | |
| NM_018950 | HLAF | 12.1 ± 1.8 | 16.7 ± 1.7 | 13.7 ± 2.0 | MHC I, F precursor |
| NM_024567 | PBHNF | 12.0 ± 1.9 | 15.3 ± 3.0 | 17.0 ± 2.8 | Homeobox containing 1 |
| NM_003764 | FHL4 | 12.0 ± 2.1 | 14.0 ± 1.4 | 11.7 ± 4.4 | Syntaxin 11 |
| THC2276547 | 11.9 ± 1.3 | 16.8 ± 4.7 | 30.9 ± 9.6 | ||
| CA431756 | CTTN | 11.3 ± 1.4 | 13.8 ± 1.5 | 12.8 ± 3.1 | |
| CR608347 | HLA-B | 11.0 ± 1.2 | 16.5 ± 1.4 | 12.7 ± 1.9 | |
| NM_005261 | KIR | 11.0 ± 4.5 | 96.2 ± 9.6 | 97.3 ± 56.7 | GTP-binding mitogen-induced T-cell protein |
| NM_130446 | FLJ00029 | 11.0 ± 1.2 | 11.6 ± 3.5 | 13.6 ± 7.1 | Kelch-like 6 |
| NM_017789 | SEMAI | 10.9 ± 1.1 | 16.4 ± 1.6 | 24.6 ± 10.4 | Semaphorin 4C |
| BC037255 | LOC389634 | 10.6 ± 1.1 | 16.8 ± 3.0 | 21.3 ± 6.4 | Hypothetical protein LOC389634 |
| AF009619 | CASH | 10.6 ± 1.7 | 16.4 ± 3.3 | 85.1 ± 38.2 | CASP8 and FADD-like apoptosis regulator |
| NM_006674 | P5-1 | 10.5 ± 1.2 | 58.0 ± 5.8 | 91.4 ± 9.3 | HLA complex P5 |
| NR_001434 | HLAHP | 10.2 ± 1.0 | 15.7 ± 1.6 | 18.5 ± 2.4 | |
a
The genes of which expression levels were more than 10-fold in all of the three HTLV-1-carrying T-cell lines (S1T, MT-2, and M8166) compared with the control T-cell line (MOLT-4) with statistical significance (P < 0.05) are listed and sorted by the expression level in S1T cells. All data represent means ± standard deviations for three independent microarray experiments.