. 2008 Feb 6;82(8):3843–3852. doi: 10.1128/JVI.02013-07

TABLE 2.

CD70 expression in PBMCs isolated from healthy donors and ATL patients^a

Donor	WBC^b (cells/mm³)	Expression on indicated marker-positive cells (%)^c
Donor	WBC^b (cells/mm³)	CD3⁺ CD70⁺	CD4⁺ CD70⁺	CD4⁺ CD25⁺	CD4⁺ CD25⁺ CD70⁺	CD8⁺ CD70⁺	CD19⁺ CD70⁺	CD14⁺ CD70⁺
HD-1		2.6	1.8	ND	0.3	0.6	16.5	0
HD-2		2.9	2.7	1.5	0.4	4.2	17.9	0.1
HD-3		2.4	1.9	3.6	0.4	0.2	13.6	0
HD-4		6.3	4.2	7.1	1.2	0.6	20.3	0.5
HD-5		9.4	5.8	7.7	1.3	3.9	15.8	0.3
HD-6		5.5	2.8	14.2	1.3	ND	27.5	0.3
ATL-1	414,000	67.5	69.2	73.6	56.6	ND	14.5	0
ATL-2	28,300	98.0	98.6	97.5	98.5	91.3	18.3	0
ATL-3	296,000	66.6	84.3	40.6	36.9	0	0	0
ATL-4	9,100	99.1	98.7	58.7	58.8	84.6	8.7	0.5
ATL-5		81.6	31.5	10.4	5.7	74.6	21.5	33.9
ATL-6		94.3	93.4	73.1	69.6	24.5	16.7	1.5

PBMCs obtained from healthy donors (HD) and acute-type ATL patients were stained with appropriate MAbs (see Materials and Methods). After staining, the cells were analyzed by laser flow cytometry.

WBC, white blood cell count.

Mean ± standard deviation values for healthy donors were 4.9 ± 2.8 for CD3⁺ CD70⁺ cells, 3.2 ± 1.5 for CD4⁺ CD70⁺ cells, 6.8 ± 4.8 for CD4⁺ CD25⁺ cells, 0.8 ± 0.5 for CD4⁺ CD25⁺ CD70⁺ cells, 1.9 ± 2.0 for CD8⁺ CD70⁺ cells, 18.6 ± 4.9 for CD19⁺ CD70⁺ cells, and 0.2 ± 0.2 for CD14⁺ CD70⁺ cells. Mean ± standard deviation values for ATL patients were 84.5 ± 14.9 for CD3⁺ CD70⁺ cells (statistically significant [P < 0.01] by t test), 79.3 ± 25.9 for CD4⁺ CD70⁺ cells (statistically significant [P < 0.01] by t test), 59.0 ± 30.3 for CD4⁺ CD25⁺ cells (statistically significant [P < 0.01] by t test), 54.4 ± 31.2 for CD4⁺ CD25⁺ CD70⁺ cells (statistically significant [P < 0.01] by t test), 55.0 ± 40.4 for CD8⁺ CD70⁺ cells, 13.3 ± 7.8 for CD19⁺ CD70⁺ cells, and 6.0 ± 13.7 for CD14⁺ CD70⁺ cells. ND, not determined.