FIG. 1.

Viral RNA loads in plasma during the vaccine phase. A stock of SIV239ΔV1-V2Δnef produced in CEMX174 cells was used for inoculation of all six monkeys on day (d) 0, and a stock of SIV239ΔV1-V2 produced in the rhesus monkey 221 cell line was used for inoculation of all six animals again on week 72 (w72). Virus containing 50 ng p27 was used for the inoculation of each animal on day 0, and virus containing 52 ng p27 was used for the inoculation of each animal at week 72. Plasma samples were obtained at the indicated times and used for quantitation of levels of viral RNA as described in Materials and Methods. The dashed line indicates the copy limit of detection for these analyses.