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ICS analysis of SIV Gag-specific responses in vaccinated and unvaccinated animals. Cryopreserved PBMC obtained 5 weeks before challenge were stimulated with an SIV Gag peptide pool and responding cells identified based on upregulation of CD69 and TNF-α. Background levels of CD69+ TNF-α+ cells in unstimulated samples ranged from 0.0% to 0.05% and were subtracted from the reported values of responding cells.
