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. 2008 Jan 30;82(8):3814–3821. doi: 10.1128/JVI.02507-07

FIG. 4.

FIG. 4.

Modulation of HNF4-dependent HBV transcription and replication by SHP. Human embryonic kidney 293T cells were transiently transfected with the HBV DNA (4.1kbp) construct plus the HNF4 expression vector and 0, 1, 2, 5, and 10 μg of the SHP expression vectors (lanes 1 to 5, respectively). (A) RNA (Northern) filter hybridization analysis of HBV transcripts. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcript was used as an internal control for RNA loading per lane. (B) DNA (Southern) filter hybridization analysis of HBV replication intermediates. HBV RC DNA, HBV relaxed circular DNA; HBV SS DNA, HBV single-stranded DNA. (C) Quantitative analysis of the 3.5-kb HBV RNA and total HBV DNA replication intermediates. The levels of the 3.5-kb HBV RNA and HBV DNA replication intermediates are reported relative to the HBV DNA (4.1-kbp) construct in the presence of HNF4 expression (lane 1), which are designated as having a relative activity of 1.0. The mean RNA and DNA levels plus standard deviations from three independent analyses are shown.