FIG. 6.
A novel ESE within exon 2 that binds selectively to SRp75. (A) Diagram of the in vitro RNA substrates used. (B) Representative in vitro splicing assays of HNE was performed with the indicated RNA substrates. Positions of the unspliced RNA precursors and spliced products are indicated on the left. (C) UV cross-linking of the indicated substrates with different amounts of HNE was performed, and the labeled proteins were resolved by polyacrylamide gel electrophoresis. (D) UV cross-linked products were immunoprecipitated with the indicated antibodies (α-16H3 and α-SRp75).