FIG. 2.

Analyses of the nuclei of Ad12-infected cells by FISH. HeLa, BHK21, or BHK21-hCAR cells were infected with Ad12 at an MOI of 500 PFU/cell. At the indicated times, nuclei were prepared and Ad12 genomes were visualized by FISH as described in the supplemental material (green channel). Photographs were taken with a Zeiss LSM4 confocal laser scan microscope. Nuclei were counterstained with 20 nM propidium iodide (red channel). The insets show magnifications of selected cells.