TABLE 4.
Microsatellite analysis of plasma samples with heterogeneous viral sequencesa
| Locus | Allele(s) identified in sample obtained (mo/day/yr) from indicated subject
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| ZM229M
|
ZM215F
|
ZM247F
|
ZM246F
|
ZM246Mc 04/04/03 | |||||
| 07/17/02 | 10/19/02 | 07/04/02 | 10/19/02 | 10/28/03 | 11/01/03 | 01/14/03 | 04/04/03b | ||
| D8S1179 | 14, 16 | 14, 16 | 13, 15 | 13, 15 | 15, 17 | 15, 17 | 14 | 14 | 14 |
| D211S11 | 30.2, 31.2 | 30.2, 31.2 | 28, 36 | 28, 36 | 28.2, 27 | 28.2, 27 | 28, 31 | 27, 28, 31 | 27, 28 |
| D7S820 | 11, 12 | 11, 12 | 8, 10 | 8, 10 | 10, 11 | 10, 11 | 10, 13 | 10, 12, 13 | 12 |
| CSF1PO | 7, 12 | 7, 12 | 10, 11 | 10, 11 | 10, 12 | 10, 12 | 7, 10 | 7, 10, 12 | 12 |
| D3S1358 | 15, 17 | 15, 17 | 17 | 17 | 16, 17 | 16, 17 | 15 | 15, 16, 17 | 16, 17 |
| TH01 | 6, 8 | 6, 8 | 7, 8 | 7, 8 | 7, 9.3 | 7, 9.3 | 7, 8 | 7, 8, 9 | 8, 9 |
| D13S317 | 12, 13 | 12, 13 | 10,12 | 10, 12 | 12, 13 | 12, 13 | 11, 12 | 11, 12, 14 | 11, 14 |
| D16S539 | 12, 13 | 12, 13 | 9, 12 | 9, 12 | 10 | 10 | 11 | 10, 11, 12 | 10, 12 |
| D2S1338 | 22, 23 | 22, 23 | 21, 22 | 21, 22 | 20 | 20 | 19, 22 | 19, 22, 23 | 19, 23 |
| D19S433 | 12, 14 | 12, 14 | 11, 13 | 11, 13 | 14, 16.2 | 14, 16.2 | 13, 14 | 13, 14, 15 | 13, 15 |
| vWA | 13, 20 | 13, 20 | 16, 17 | 16, 17 | 18 | 18 | 15 | 15 | 15 |
| TPOX | 9, 11 | 9, 11 | 10, 11 | 10, 11 | 9, 11 | 9, 11 | 7, 11 | 7, 8, 11 | 8, 11 |
| D18S51 | 14.2, 16 | 14.2, 16 | 16, 18 | 16, 18 | 17, 20 | 17, 20 | 17, 18 | 16, 17, 18 | 16 |
| D5S818 | 12 | 12 | 13 | 13 | 12, 14 | 12, 14 | 12, 13 | 11, 12, 13 | 11, 12 |
| FGA | 20, 22 | 20, 22 | 21, 26 | 21, 26 | 24, 28 | 24, 28 | 22, 23 | 22, 23 | 22, 23 |
| Amelogenin | Y | Y | X | X | X | X | X | X, Y | Y |
Microsatellite analyses were performed using DNA extracted from plasma samples, except for subject ZM246M for whom only peripheral blood mononuclear cell DNA was available. Loci were amplified using an AmpFlSTR Identifiler kit (Applied Biosystems, Foster City, CA). This PCR amplification kit is a short tandem repeat multiplex assay that amplifies 15 tetranucleotide repeat loci and the amelogenin gender-determining marker in a single PCR amplification; all identifiable alleles per locus are shown.
This sample was identified as an inadvertent mixture of plasma from two different individuals (ZM246F and ZM246M).
Subject ZM246M is the chronically infected partner of subject ZM246F but not the individual who transmitted virus to subject ZM246F (see Fig. 7 and associated text for explanation).