FIG. 5.

In vitro neutralization activity does not predict complement lysis activity. Lysis activities (shown as bars) of MAbs 2G12, 2F5, and 4E10 and the mix of these three antibodies against the 14 patient virus isolates do not show a common pattern. Neutralization activities (IC₉₀ values, inverse logarithmic scale) of MAbs 2G12 (circles), 2F5 (triangle), and 4E10 (diamonds) and the mix of the three MAbs (open squares) in a PBMC-based neutralization assay against each virus is depicted above the corresponding bars. The IC₉₀s of the individual MAbs and the equimolar mix of the MAbs were determined using a PBMC-based neutralization assay (61). Error bars show standard deviations of triplicate measurements.