TABLE 1.
Sequences, chromosomal locations, and cloning frequencies of chicken miRNAs cloned from an MSB-1 library
| Namea | Sequence | No. of hits in library | Chromosomal location | Start position | End position |
|---|---|---|---|---|---|
| gga-mir-7 | TGGAAGACTAGTGATTTTGTTG | 3 | Z_random | 12717978 | 12718086 |
| gga-mir-15a | TAGCAGCACATAATGGTTTGT | 28 | 1 | 161540787 | 161540869 |
| gga-mir-15b | TAGCAGCACATCATGGTTTGCA | 9 | 9 | 21649291 | 21649381 |
| 1 | 161540645 | 161540728 | |||
| gga-mir-16 | TAGCAGCACGTAAATATTGGTG | 82 | 9 | 21649116 | 21649209 |
| gga-mir-17-5p | CAAAGTGCTTACAGTGCAGGTAA | 55 | 1 | 140631124 | 140631208 |
| gga-mir-18a | TAAGGTGCATCTAGTGCAGATA | 10 | 1 | 140630969 | 140631061 |
| gga-mir-18b | TAAGGTGCATCTAGTGCAGTTA | 10 | 4 | 3781954 | 3782037 |
| gga-mir-19a | TGTGCAAATCTATGCAAAACTGA | 71 | 1 | 140630835 | 140630915 |
| gga-mir-19b | TGTGCAAATCCATGCAAAACTGA | 71 | 1 | 140630526 | 140630612 |
| gga-mir-20a | TAAAGTGCTTATAGTGCAGGTAG | 18 | 1 | 140630649 | 140630746 |
| gga-mir-20b | CAAAGTGCTCATAGTGCAGGTAG | 16 | 4 | 3781773 | 3781857 |
| gga-mir-21 | TAGCTTATCAGACTGATGTTGA | 249 | 19 | 6933581 | 6933677 |
| gga-mir-23b | ATCACATTGCCAGGGATTAC | 2 | Z_random | 14203199 | 14203284 |
| gga-mir-24 | TGGCTCAGTTCAGCAGGAACAG | 6 | Z_random | 14203968 | 14204035 |
| gga-mir-26a | TTCAAGTAATCCAGGATAGGC | 14 | 2 | 4034213 | 4034289 |
| gga-mir-27b | TTCACAGTGGCTAAGTTCTGC | 10 | Z_random | 14203435 | 14203531 |
| 26 | 1280249 | 1280328 | |||
| gga-mir-29b | TAGCACCATTTGAAATCAGTGTT | 98 | 1 | 204569 | 204649 |
| gga-mir-30b | TGTAAACATCCTACACTCAGCT | 1 | 2 | 141145952 | 141146038 |
| 3 | 80699454 | 80699525 | |||
| gga-mir-30c | TGTAAACATCCTACACTCTCAGCT | 1 | 23 | 4431115 | 4431203 |
| gga-mir-30d | TGTAAACATCCCCGACTGGAAGC | 16 | 2 | 141142201 | 141142264 |
| gga-mir-30a-5p | TGTAAACATCCTCGACTGGAAGCT | 13 | 3 | 80674840 | 80674911 |
| gga-mir-33 | GTGCATTGTAGTTGCATTG | 6 | 1 | 46203134 | 46203202 |
| gga-mir-34a | TGGCAGTGTCTTAGCTGGTTGTT | 7 | 21 | 3118925 | 3119033 |
| gga-miR-92 | TATTGCACTTGTCCCGGCCTGT | 9 | 1 | 140630413 | 140630490 |
| gga-mir-101 | TACAGTACTGTGATAACTGAAG | 9 | Z | 11651019 | 11651097 |
| gga-mir-106 | AAAAGTGCTTACAGTGCAGGTA | 55 | 4 | 3782085 | 3782165 |
| gga-mir-130a | CAGTGCAATATTAAAAGGGCA | 4 | 15 | 393029 | 393111 |
| gga-mir-140 | AGTGGTTTTACCCTATGGTAG | 5 | 11_random | 250924 | 251018 |
| gga-mir-142-3p | TGTAGTGTTTCCTACTTTATGGA | 224 | Un | 130069949 | 130070036 |
| gga-mir-142-5p | CCCATAAAGTAGAAAGCACTAC | 243 | Un | 130069949 | 130070036 |
| gga-mir-146b | TGAGAACTGAATTCCATAGGCG | 44 | 6 | 22212586 | 22212690 |
| gga-mir-181a | AACATTCAACGCTGTCGGTGAGTT | 5 | 8 | 1957561 | 1957664 |
| 17 | 945791 | 945881 | |||
| 8 | 1957750 | 1957838 | |||
| gga-mir-181b | AACATTCATTGCTGTCGGTGGGTTT | 6 | 17 | 944157 | 944241 |
| gga-mir-221 | AGCTACATTGTCTGCTGGGTTTC | 11 | 1 | 104369325 | 104369423 |
| gga-mir-222 | AGCTACATCTGGCTACTGGGT | 7 | 1 | 104368821 | 104368918 |
| gga-mir-301 | CAGTGCAATAATATTGTCAAAGCATT | 3 | 15 | 391803 | 391895 |
| gga-mir-456 | CAGGCTGGTTAGATGGTTGTCCT | 4 | 3 | 27735429 | 27735540 |
| gga-let-7i | TGAGGTAGTAGTTTGTGCTGT | 148 | 1 | 29988296 | 29988379 |
| 12 | 6149732 | 6149821 | |||
| gga-let-7a | TGAGGTAGTAGGTTGTATAGTT | 12 | 24 | 3263054 | 3263125 |
| 1 | 67795667 | 67795742 | |||
| gga-mir-363* | AATTGCACGGTATCCATCTGTA | 30 | |||
| gga-mir-454* | TAGTGCAATATTGCTTATAGGGT | 5 | |||
| gga-mir-425* | AATGACACGATCACTCCCGTTGA | 6 | |||
| gga-mir-191* | CAACGGAATCCCAAAAGCAGCTG | 8 | |||
| gga-mir-22* | AAGCTGCCAGTTGAAGAACTGT | 6 | |||
| gga-mir-739* | AAGGCCGAAGTGGAGAAGGGTTCCA | 1 |
a
*, novel miRNAs identified in chickens and assigned names on the basis of homology to miRNAs in other species.