TABLE 1.
Constrained CCR5 peptides used in this study and amino acid sequences in ECL1 of CCR5 in the different species
| Peptide | Sequencea |
|---|---|
| Human WT cyclic peptide | kgcYAAAQWDFGNTMCQgk |
| Gly 89 | kgcG-------------gk |
| Gly 90 | kgc-G------------gk |
| Gly 91 | kgc--G-----------gk |
| Gly 92 | kgc---G----------gk |
| Gly 93 | kgc----G---------gk |
| Ala 94 | kgc-----A--------gk |
| Ala 95 | kgc------A-------gk |
| Ala 96 | kgc-------A------gk |
| Ala 97 | kgc--------A-----gk |
| Ala 98 | kgc---------A----gk |
| Ala 99 | kgc----------A---gk |
| Ala 100 | kgc-----------A--gk |
| Ala 95-96 | kgc------AA------gk |
| Human ECL1 | YAAAQWDFGNTMCQ |
| Murine ECL 1 | ---NE-V---I--K |
| Chicken ECL 1 | ---HD-I-DALCR |
a
Small letters indicate extrasequence amino acids introduced to allow the etherocyclization of the peptides and to allow them to be coupled to Dynabeads.