FIG. 7.

Synthesis and processing of viral proteins. The synthesis of viral proteins was determined by Western blotting of lysates from cells harvested at the indicated hours postinfection (hpi) with vIHD-J or vIHD-JΔE2L. (Top) Blot probed with antibodies induced by infection of rabbits with live VACV. Positions of marker proteins are shown on the left. (Bottom) Blots probed with antibodies to peptides derived from the sequence of the A3 (core), A17 (MV membrane), B5 (EV membrane), and E2 proteins. The antibodies A17n and A17c target the N terminus of the mature A17 protein and the C terminus of the A17 precursor protein, respectively.