TABLE 3.
Severity of histological lesions and distribution of viral antigen in tissues from chickens IN inoculated with recombinant AIVsa
| Tissue | Histological lesions/viral antigen stainingb
|
|||||||
|---|---|---|---|---|---|---|---|---|
| rEgret | rIndo | rIndo/Egret HA | rIndo/Egret NS | rIndo/Egret M | rIndo/Egret NP | rIndo/Egret PB2 | rIndo/Egret-PB1 | |
| Trachea | ++/+ | ++/+ | ++/+ | ++/+ | ++/+ | +++/++ | +/− | +/− |
| Lung | ++/++ | ++/++ | ++/++ | ++/++ | ++/+ | +++/+++ | +/+ | +/+ |
| Heart | +/++ | −/− | +/+ | +/+ | +/++ | ++/+++ | −/− | −/− |
| Brain | +/++ | −/+ | +/+ | ++ | −/− | ++/+++ | −/− | −/− |
| Pancreas | +/+ | −/− | +/+ | −/− | −/+ | ++/++ | −/− | −/− |
| Adrenal | +/++ | −/− | −/− | −/− | −/− | ++/++ | −/− | −/− |
| Intestine | −/+ | −/− | −/− | −/− | −/− | +/+ | −/− | −/− |
| Liver | −/++ | −/+ | −/+ | −/+ | −/− | +/+ | −/− | −/− |
| Kidney | +/+ | −/− | +/+ | +/+ | −/+ | +/++ | −/− | −/− |
| Spleen | +/++ | −/++ | +/++ | ++/++ | +/+ | +++/+++ | −/− | −/− |
| Bursa | ++/+ | −/− | −/− | −/− | −/− | ++/+ | −/− | −/− |
| Thymus | +/+ | −/− | −/+ | +/+ | −/+ | +/++ | −/− | −/− |
| Muscle | −/+ | −/− | −/− | −/− | −/− | +/++ | −/− | −/− |
| Gizzard | −/+ | −/− | −/− | −/− | −/− | +/++ | −/− | −/− |
a
Tissues were taken 2 dpi and were immunohistochemically stained with antibodies to AIV nucleoprotein to visualize the viral antigen.
b
Lesions were scored as follows: −, no lesions; +, mild; ++, moderate; +++, severe lesions. The intensity of viral antigen staining in each section was scored as follows: −, no antigen staining; +, infrequent; ++, common; +++, widespread staining.