FIG. 1.

The Nsp1 C-terminal region is important for inhibition of reporter gene activity. (A) 293 cells were independently cotransfected with pRL-SV40 and pCAGGS (EV), pRL-SV40 and pCAGGS-Nsp1-WT (Nsp1 WT), or pRL-SV40 and pCAGGS-Nsp1Δ160-173 (Nsp1Δ160-173). (A) Total intracellular proteins were extracted at 30 h posttransfection, and Western blot analysis was performed using anti-myc antibody. (B) At 30 h after transfection, cells were lysed in Renilla luciferase lysis buffer, and aliquots of lysates were used to measure rLuc activities.