Skip to main content
. 2008 Feb 27;82(9):4471–4479. doi: 10.1128/JVI.02472-07

FIG. 2.

FIG. 2.

Mutations in the C-terminal region of Nsp1 abrogate its ability to inhibit reporter gene expression. (A) 293 cells were transfected with pCAGGS (EV), pCAGGS-Nsp1-WT (WT), or pCAGGS-Nsp1-mt (mt). At 30 h posttransfection, total intracellular proteins were extracted and Western blot analysis was performed using anti-myc antibody. (B) 293 cells were cotransfected with pIFNβ-luc and pCAGGS (EV), pIFNβ-luc and pCAGGS-Nsp1-WT (WT), or pIFNβ-luc and pCAGGS-Nsp1-mt (mt). At 24 h posttransfection, cells were infected with SeV (+) or mock infected (-). At 16 h p.i., firefly luciferase (Luc) activities were measured. (C and D) 293 cells were independently cotransfected with pRL-SV40 and pCAGGS (EV), pRL-SV40 and pCAGGS-Nsp1-WT (WT), or pRL-SV40 and pCAGGS-Nsp1-mt (mt). At 30 h posttransfection, cell extracts were prepared and used to measure rLuc activities (C), or total RNAs were extracted and Northern blot analysis was performed using a riboprobe specific for rLuc (D).