FIG. 5.

Effect of SARS-CoV-mt replication on abundance of host endogenous mRNAs. 293/ACE2 cells (A and B) and Vero E6 cells (A and C) were either mock infected or infected with SARS-CoV-WT (WT) or SARS-CoV-mt (mt) at an MOI of 3. (A) Intracellular RNAs were extracted at 1 and 16 h p.i. The amount of endogenous GAPDH mRNA was determined by Northern blot analysis. (B and C) At 1 h p.i., intracellular RNAs were extracted (1 h) or ActD was added to the culture medium. Intracellular RNAs were extracted at 15 h after ActD addition (16 h). The abundance of GAPDH (B, upper panel) and β-actin (B, lower panel, and C) mRNAs was determined using Northern blot analysis.