FIG. 1.
Insert sequence and one-step growth curve of rCVB3.6. (A) The N-terminal sequence of rCVB3.6 is shown, with the LCMV GP33-41 and GP61-80 epitopes (residues 8 to 16 and 21 to 40, respectively) underlined and separated by a polyglycine linker. The epitopes are followed by an artificial CVB3 3C viral proteinase recognition site (ALFQG, dashed underline) that, when cleaved (at position ▾), releases the N-terminal polypeptide from the CVB3 polyprotein. (B) One-step growth curves of wtCVB3 and rCVB3.6. HeLa cell monolayers were infected at a multiplicity of infection of 20, and the virus titer was determined at the indicated time points by plaque assay. Data are representative of two independent experiments.