TABLE 2.
Binding affinities of CP(81-112) alanine mutant peptides for encapsidation signal RNA
| CP peptide | Avg Kdapp (nM)a |
|---|---|
| Wild type | 12 ± 2 |
| K83A | 12 ± 1 |
| K86A | 9 ± 1 |
| Q88A | 14 ± 2 |
| K92A | 19 ± 2 |
| Q94A | 13 ± 3 |
| K97A | 13 ± 2 |
| K99A | >100 |
| K99L | >100 |
| K99M | 10 ± 2 |
| K99R | 8 ± 2 |
| K99E | >100 |
| P100A | 14 ± 2 |
| K102A | 8 ± 2 |
| R103A | 8 ± 2 |
| Q104A | 13 ± 3 |
| R105A | >100 |
| R105L | >100 |
| R105M | >100 |
| R105K | 9 ± 2 |
| R105E | >100 |
| L108A | 4 ± 2 |
| K109A | 10 ± 1 |
| L110A | 10 ± 1 |
| K97A/K99A | >100 |
| K99A/R105A | >100 |
| R103A/R105A | >1000 |
a
Kdapp, apparent dissociation constant. Values are means ± standard deviations.