Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Purified US3 and UL13 kinases phosphorylate ICP22 and cdc25C. GST-US3 or GST-US3-M was mixed with GST-UL13 or GST-UL13-M in various combinations and added to MBP-ICP22 or MBP-cdc25C-M bound to beads in the presence of [γ-32P]ATP for 30 min. Beads were rinsed and proteins separated by electrophoresis, transferred, and analyzed by autoradiography (A). The membrane was stained with Ponceau S to detect total protein (D), immunoblotted first for GST (B), and then reprobed for MBP (C).
