Sequence feature of ISKNV ORF48R. (A) Alignment of the VEGF homologues of human, rat, zebrafish, ISKNV, RBIV, OSGIV, LYCIV, ORFV, PCPV, BPSV, and PVNZ. Black shading indicates 100% identity, medium-gray shading shows 80% to 100% identity, and light-gray shading shows 60% to 80% identities. (B) Phylogenetic tree of VEGF-like sequences. The diverse VEGF sequences were analyzed using the ClustalW program. The phylogenetic tree was constructed using the Bootstrap N-J method of Phylip 3.63 programs, and the tree was output using the program TreeView 1.6.6 (50). The bootstrap test of phylogeny was calculated with 1,000 replicates. Abbreviations: Hs, Homo sapiens; Ms, Mus musculus; Dr, Danio rerio; Sh, sheep; Ca, cattle; Me, Medaka; Rt, rainbow trout; PVH, predicted VEGF homologues.