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. 2008 Feb 27;82(9):4371–4383. doi: 10.1128/JVI.02027-07

FIG. 4.

FIG. 4.

Quantitative PCR analysis of the expression of vascular endothelial and hematopoietic cell marker genes induced by pG48R in the zebrafish embryonic development. Expression of tie1, flk1, gata1, and scl at the zebrafish developmental stages at 24 hpf (A), 48 hpf (B), 72 hpf (C), and 4 dpf (D). The black column represents the average expression value of the marker genes in wild-type embryos. The gray column represents the average expression value of the marker genes in pcXGFP-injected embryos. The white column represents the average expression of the marker genes in pG48R-injected embryos. The expression level of β-actin was set as 1. Results of relative quantification were analyzed with the method of Liu and Saint (37).