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. 2008 Feb 27;82(9):4371–4383. doi: 10.1128/JVI.02027-07

FIG. 5.

FIG. 5.

RNA in situ hybridization of flk1 expression induced by pG48R during early embryogenesis. Embryos shown in panels A to C were at 24 hpf. Embryos shown in panels D to H were at 48 hpf. At 24 hpf, compared to the wild-type embryos (A) and pcXGFP-injected embryos (B), the flk1 expression in pG48R-injected embryos (C) was enhanced in the dorsal aorta (A to C, arrowhead) and the tail region (A to C, arrow). At 48 hpf, the position and up-regulated expression of flk1 (F, arrowhead and arrow) in pG48R-injected embryos were similar to those in pGV121-injected embryos (G, arrowhead and arrow) and pG48R-pGV121-coinjected embryos (H, arrowhead and arrow), compared to the wild-type embryos (D) and pcXGFP-injected embryos (E). In addition, the up-regulated expression of flk1 in the endocardium was also observed (F, large arrow). Embryos shown in all panels are lateral views with anterior to the left. Bars, 300 μm (A to C) and 500 μm (D, E, F, G, and H).